Composite

Part:BBa_K3778029

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-09-05)


T7P-RBS-6xHis-CBD-linker-LVPRFA-eGFP-T7T

The synthetic protein from this composite part is applied to examine the thrombin releasing rate of D2A21 (BBa_K3778007).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 729
    Illegal NheI site found at 1491
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 729
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None